Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Published in Review of Scientific Instruments, 2018

Recommended citation: Stadnytskyi et. al. Review of Scientific Instruments 89, 033104 (2018) https://doi.org/10.1063/1.5009468

We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of ╬╝deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. The spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.

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Recommended citation: Stadnytskyi et. al. Review of Scientific Instruments 89, 033104 (2018)